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aav2 reference standard material  (ATCC)
95
ATCC aav2 reference standard material
Figure 1. TCID50 workflow with qPCR and ddPCR as an endpoint measurement. TCID50 is a cell-based assay to measure infectious virus titer. The assay workflow is as follows: (A) Day 0: cell plating. HeLaRC32 cells were plated in 96-well plate at the density of 4.0E+05 cells/mL. (B) Day 1: infection. Twenty-four hours after cell plating, series of diluted viral vectors were added to the wells accordingly, along with Ad5 helper virus; the infection plate layout is explained in Supplementary Table S1 (gradient blue code is for serial dilution of AAV-2 co-infected with Ad5, green is for Ad5 control-only wells, and gray is for UI wells. (C) Day 3: cell lysis. Forty-eight hours after infection, cells were lysed using Proteinase K solution; Day 3–9: endpoint detection. Cell lysate was diluted 1:50 or 1:100 in TE buffer before use with ddPCR or qPCR runs. The results were then analyzed according to the Spearman-Ka¨rber method to calculate the TCID50 in terms of infectious titer (in IU/mL) and specific infectivity (vg/IU). AAV-2, adeno-associated virus-2; ddPCR, droplet digital PCR; IU, infectious unit; qPCR, quantitative PCR; TCID50, 50% tissue culture infective dose; TE, Tris-EDTA buffer; vg, viral genome.
Aav2 Reference Standard Material, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/video-based+real-time+position+management+system+rpm/Recombinant+Adeno-associated+virus+2/pm37276150-47-1-10
Average 95 stars, based on 1 article reviews
aav2 reference standard material - by Bioz Stars, 2026-10
95/100 stars
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video-based sarcomere-detection system  (IonOptix)
90
IonOptix video-based sarcomere-detection system
Figure 1. TCID50 workflow with qPCR and ddPCR as an endpoint measurement. TCID50 is a cell-based assay to measure infectious virus titer. The assay workflow is as follows: (A) Day 0: cell plating. HeLaRC32 cells were plated in 96-well plate at the density of 4.0E+05 cells/mL. (B) Day 1: infection. Twenty-four hours after cell plating, series of diluted viral vectors were added to the wells accordingly, along with Ad5 helper virus; the infection plate layout is explained in Supplementary Table S1 (gradient blue code is for serial dilution of AAV-2 co-infected with Ad5, green is for Ad5 control-only wells, and gray is for UI wells. (C) Day 3: cell lysis. Forty-eight hours after infection, cells were lysed using Proteinase K solution; Day 3–9: endpoint detection. Cell lysate was diluted 1:50 or 1:100 in TE buffer before use with ddPCR or qPCR runs. The results were then analyzed according to the Spearman-Ka¨rber method to calculate the TCID50 in terms of infectious titer (in IU/mL) and specific infectivity (vg/IU). AAV-2, adeno-associated virus-2; ddPCR, droplet digital PCR; IU, infectious unit; qPCR, quantitative PCR; TCID50, 50% tissue culture infective dose; TE, Tris-EDTA buffer; vg, viral genome.
Video Based Sarcomere Detection System, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/video-based+real-time+position+management+system+rpm/video+based+sarcomere+length+detection+system/pm24610916-61-18-22
Average 90 stars, based on 1 article reviews
video-based sarcomere-detection system - by Bioz Stars, 2026-10
90/100 stars
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modelling real time video processing system  (MathWorks Inc)
96
MathWorks Inc modelling real time video processing system
Figure 1. TCID50 workflow with qPCR and ddPCR as an endpoint measurement. TCID50 is a cell-based assay to measure infectious virus titer. The assay workflow is as follows: (A) Day 0: cell plating. HeLaRC32 cells were plated in 96-well plate at the density of 4.0E+05 cells/mL. (B) Day 1: infection. Twenty-four hours after cell plating, series of diluted viral vectors were added to the wells accordingly, along with Ad5 helper virus; the infection plate layout is explained in Supplementary Table S1 (gradient blue code is for serial dilution of AAV-2 co-infected with Ad5, green is for Ad5 control-only wells, and gray is for UI wells. (C) Day 3: cell lysis. Forty-eight hours after infection, cells were lysed using Proteinase K solution; Day 3–9: endpoint detection. Cell lysate was diluted 1:50 or 1:100 in TE buffer before use with ddPCR or qPCR runs. The results were then analyzed according to the Spearman-Ka¨rber method to calculate the TCID50 in terms of infectious titer (in IU/mL) and specific infectivity (vg/IU). AAV-2, adeno-associated virus-2; ddPCR, droplet digital PCR; IU, infectious unit; qPCR, quantitative PCR; TCID50, 50% tissue culture infective dose; TE, Tris-EDTA buffer; vg, viral genome.
Modelling Real Time Video Processing System, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/video-based+real-time+position+management+system+rpm/Simulink+Real-Time/10__1111_slash_exsy__12749-245-7-13
Average 96 stars, based on 1 article reviews
modelling real time video processing system - by Bioz Stars, 2026-10
96/100 stars
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infrared video-based eye tracker eyelink ii  (SR Research)
90
SR Research infrared video-based eye tracker eyelink ii
Figure 1. TCID50 workflow with qPCR and ddPCR as an endpoint measurement. TCID50 is a cell-based assay to measure infectious virus titer. The assay workflow is as follows: (A) Day 0: cell plating. HeLaRC32 cells were plated in 96-well plate at the density of 4.0E+05 cells/mL. (B) Day 1: infection. Twenty-four hours after cell plating, series of diluted viral vectors were added to the wells accordingly, along with Ad5 helper virus; the infection plate layout is explained in Supplementary Table S1 (gradient blue code is for serial dilution of AAV-2 co-infected with Ad5, green is for Ad5 control-only wells, and gray is for UI wells. (C) Day 3: cell lysis. Forty-eight hours after infection, cells were lysed using Proteinase K solution; Day 3–9: endpoint detection. Cell lysate was diluted 1:50 or 1:100 in TE buffer before use with ddPCR or qPCR runs. The results were then analyzed according to the Spearman-Ka¨rber method to calculate the TCID50 in terms of infectious titer (in IU/mL) and specific infectivity (vg/IU). AAV-2, adeno-associated virus-2; ddPCR, droplet digital PCR; IU, infectious unit; qPCR, quantitative PCR; TCID50, 50% tissue culture infective dose; TE, Tris-EDTA buffer; vg, viral genome.
Infrared Video Based Eye Tracker Eyelink Ii, supplied by SR Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/video-based+real-time+position+management+system+rpm/eyelink+1000/pmc07202594-46-12-16
Average 90 stars, based on 1 article reviews
infrared video-based eye tracker eyelink ii - by Bioz Stars, 2026-10
90/100 stars
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Figure 1. TCID50 workflow with qPCR and ddPCR as an endpoint measurement. TCID50 is a cell-based assay to measure infectious virus titer. The assay workflow is as follows: (A) Day 0: cell plating. HeLaRC32 cells were plated in 96-well plate at the density of 4.0E+05 cells/mL. (B) Day 1: infection. Twenty-four hours after cell plating, series of diluted viral vectors were added to the wells accordingly, along with Ad5 helper virus; the infection plate layout is explained in Supplementary Table S1 (gradient blue code is for serial dilution of AAV-2 co-infected with Ad5, green is for Ad5 control-only wells, and gray is for UI wells. (C) Day 3: cell lysis. Forty-eight hours after infection, cells were lysed using Proteinase K solution; Day 3–9: endpoint detection. Cell lysate was diluted 1:50 or 1:100 in TE buffer before use with ddPCR or qPCR runs. The results were then analyzed according to the Spearman-Ka¨rber method to calculate the TCID50 in terms of infectious titer (in IU/mL) and specific infectivity (vg/IU). AAV-2, adeno-associated virus-2; ddPCR, droplet digital PCR; IU, infectious unit; qPCR, quantitative PCR; TCID50, 50% tissue culture infective dose; TE, Tris-EDTA buffer; vg, viral genome.

Journal: Human gene therapy

Article Title: Improvement of Precision in Recombinant Adeno-Associated Virus Infectious Titer Assay with Droplet Digital PCR as an Endpoint Measurement.

doi: 10.1089/hum.2023.014

Figure Lengend Snippet: Figure 1. TCID50 workflow with qPCR and ddPCR as an endpoint measurement. TCID50 is a cell-based assay to measure infectious virus titer. The assay workflow is as follows: (A) Day 0: cell plating. HeLaRC32 cells were plated in 96-well plate at the density of 4.0E+05 cells/mL. (B) Day 1: infection. Twenty-four hours after cell plating, series of diluted viral vectors were added to the wells accordingly, along with Ad5 helper virus; the infection plate layout is explained in Supplementary Table S1 (gradient blue code is for serial dilution of AAV-2 co-infected with Ad5, green is for Ad5 control-only wells, and gray is for UI wells. (C) Day 3: cell lysis. Forty-eight hours after infection, cells were lysed using Proteinase K solution; Day 3–9: endpoint detection. Cell lysate was diluted 1:50 or 1:100 in TE buffer before use with ddPCR or qPCR runs. The results were then analyzed according to the Spearman-Ka¨rber method to calculate the TCID50 in terms of infectious titer (in IU/mL) and specific infectivity (vg/IU). AAV-2, adeno-associated virus-2; ddPCR, droplet digital PCR; IU, infectious unit; qPCR, quantitative PCR; TCID50, 50% tissue culture infective dose; TE, Tris-EDTA buffer; vg, viral genome.

Article Snippet: rAAV, AAV2 reference standard material (AAV2 rss) was purchased from ATCC (Cat. No. VR-1616).22,23 Human adenovirus 5 (Ad5) was obtained from ATCC (Cat. No. VR-1516).

Techniques: Cell Based Assay, Virus, Infection, Serial Dilution, Control, Lysis, Digital PCR, Real-time Polymerase Chain Reaction